ICAM-1-IN-1

Name: ICAM-1-IN-1
Brand: MedChemExpress
SKU: HY-U00003
Rating: 4.5 (1 reviews)

Cat. No.: HY-U00003 Purity: 99.94%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

ICAM-1-IN-1 is a potent and selective inhibitor of E-selectin and ICAM-1 with IC₅₀ values of 7 and 5 nM, respectively.

For research use only. We do not sell to patients.

ICAM-1-IN-1 Chemical Structure

CAS No. : 251994-14-6

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 550	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 550	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 238	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 850	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1800	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 3000	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 4600	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Protocol
Purity & Documentation
References
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Description

ICAM-1-IN-1 is a potent and selective inhibitor of E-selectin and ICAM-1 with IC₅₀ values of 7 and 5 nM, respectively.

IC₅₀ & Target

IC50: 7 nM (E-selectin), 5 nM (ICAM-1)^[1]

In Vivo

ICAM-1-IN-1 shows significant efficacy in a rat rheumatoid arthritis model and in a mouse asthma model. ICAM-1-IN-1 reduces the width of inflamed ankles after the ninth day following treatment, but shows no efficacy in the acute phase. Treatment with ICAM-1-IN-1 (25 mg/kg) for 21 days significantly reduces the ankle inflammation of the arthritis rats. Significant reduction of eosinophils and serum-soluble ICAM-1 (sICAM-1) is observed^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

363.23

Formula

C₁₅H₁₁BrN₂O₂S

CAS No.

251994-14-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(C(S1)=CC2=C1C=NC=C2OC3=CC=C(Br)C=C3)NC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 135 mg/mL (371.67 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7531 mL	13.7654 mL	27.5308 mL
5 mM	0.5506 mL	2.7531 mL	5.5062 mL
10 mM	0.2753 mL	1.3765 mL	2.7531 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.25 mg/mL (6.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.25 mg/mL (6.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.25 mg/mL (6.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (274 KB) SDS (0 KB)

COA (249 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Zhu GD, et al. Selective inhibition of ICAM-1 and E-selectin expression in human endothelial cells. 2. Arylmodifications of 4-(aryloxy)thieno[2,3-c]pyridines with fine-tuning at C-2 carbamides. J Med Chem. 2001 Oct 11;44(21):3469-87. [Content Brief]

Animal Administration
^[1]

Rats: In a rat rheumatoid arthritis model, 26 all rats are treated with peptidoglycan polysaccharide to elicit the disease to a desired degree. Six rats per group are then treated with ICAM-1-IN-1 or vehicle control twice daily. The ankles of these rats are measured on day 0, 1, 3, 7, 9, 11, 16, 18, and 21 after administration. Histologic analyses of the ankles are performed on the last day of the experiment^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Zhu GD, et al. Selective inhibition of ICAM-1 and E-selectin expression in human endothelial cells. 2. Arylmodifications of 4-(aryloxy)thieno[2,3-c]pyridines with fine-tuning at C-2 carbamides. J Med Chem. 2001 Oct 11;44(21):3469-87. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7531 mL	13.7654 mL	27.5308 mL	68.8269 mL
	5 mM	0.5506 mL	2.7531 mL	5.5062 mL	13.7654 mL
	10 mM	0.2753 mL	1.3765 mL	2.7531 mL	6.8827 mL
	15 mM	0.1835 mL	0.9177 mL	1.8354 mL	4.5885 mL
	20 mM	0.1377 mL	0.6883 mL	1.3765 mL	3.4413 mL
	25 mM	0.1101 mL	0.5506 mL	1.1012 mL	2.7531 mL
	30 mM	0.0918 mL	0.4588 mL	0.9177 mL	2.2942 mL
	40 mM	0.0688 mL	0.3441 mL	0.6883 mL	1.7207 mL
	50 mM	0.0551 mL	0.2753 mL	0.5506 mL	1.3765 mL
	60 mM	0.0459 mL	0.2294 mL	0.4588 mL	1.1471 mL
	80 mM	0.0344 mL	0.1721 mL	0.3441 mL	0.8603 mL
	100 mM	0.0275 mL	0.1377 mL	0.2753 mL	0.6883 mL

ICAM-1-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ICAM-1-IN-1251994-14-6IntegrinInhibitorinhibitorinhibit

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ICAM-1-IN-1

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ICAM-1-IN-1 Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Integrin Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

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Dilution Calculator

Complete Stock Solution Preparation Table

ICAM-1-IN-1 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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